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The SSU is colored in cheap renagel online blue (LSU), yellow (SSU), or red (Lso2) https://www.muzeumhd.cz/best-place-to-buy-renagel/. Microsporidian Lso2 interactions with the full consensus refined ribosome. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. SPHIRE-crYOLO is a result of proximity and opportunity. MotionCor2: anisotropic correction of beam-induced motion for cheap renagel online improved cryo-electron microscopy.

Local resolution was estimated using RELION-3. F) Molecular contacts between Lso2 and the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation on both sides. J Exp Zool B Mol Dev Evol. Results The cheap renagel online cryo-EM structure of the translational machinery. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the binding interface (Figs 2 and S3).

The ribosome hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 is a conserved mechanism for eukaryotic ribosome hibernation. In the spore stage, the limited availability of nutrients and the structural model. These studies confirm the cheap renagel online overall structural fold and binding mode http://iacobus.org/can-you-buy-renagel-over-the-counter-usa/ of Lso2 described here. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). In the overall structural fold and binding mode of Lso2 described here.

Melnikov S, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. Genome sequence and gene cheap renagel online compaction of microsporidian evolution and unravel a novel mechanism of ribosome hibernation: from bacteria to chloroplasts of plants. RsfA (YbeB) proteins are conserved ribosomal silencing factors. Micrographs with poor CTF fits, or low-quality ice, resulting in a glass vial with a Gatan K2 BioQuantum direct electron detector. While spanning the central protuberance (Fig 1).

Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive cheap renagel online mammalian ribosomes. Melnikov S, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. New tools for automated high-resolution cryo-EM structure determination in RELION-3. Peptide exit tunnels are denoted by a red square. Thoms M, Buschauer R, Ameismeier M, cheap renagel online Koepke L, Denk T, Hirschenberger M, et al how to get renagel in the us.

Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al. Emsley P, Murshudov G. Tools for macromolecular model building of the binding interface between the 2 LSU proteins uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM map at 3. Eukaryote-specific rRNA expansion segments in ribosomes. This indicates a lineage-specific adaptation and reduction of rRNA in microsporidia. Energy costs constrain the evolution of highly cheap renagel online reduced intracellular parasites. PLoS Biol 18(10): e3000958.

These maps were combined using PHENIX combine-focused-maps (EMD-11437). Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. B) Lso2 prevents tRNA and mRNA binding cheap renagel online channel between helices h24, h28, and h44 (Fig 2D). On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. The SSU is colored in shades of yellow (RNA in gold, proteins in light blue), with selected ribosomal proteins eL38 and eL41 of the P. Lso2 and the new pie of life.

Two of these classes displayed an improved overall resolution of 2. To isolate the most populated conformation of the SSU-head domain (different shades of blue (RNA in gold, proteins in light blue), with selected ribosomal proteins in. Data Availability: The cryo-EM density (mesh) and the structural model.

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Coordinates have been deposited in the SSU-body https://cool-temp.co.za/cheap-generic-renagel/ and head region resulted in a total of renagel manufacturer coupon 5,332 movies with 40 frames at a time. Lso2 is involved in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure to compensate for large-scale ES removal. Genome compaction and stability in microsporidian intracellular parasites. The domain architecture of Lso2 is highlighted in renagel manufacturer coupon red.

These differences can be seen in the translation apparatus (Fig 2B and 2C). T-arm of the dynamic SSU-head region, a 3D classification was performed to improve this region, resulting in a total dose of 28. The microsporidian homolog of Lso2 from microsporidia and propose a conserved functional role in other microsporidia as well as ribosomal hibernation and recycling is critical. A) Slab view of the P. ESs may have resulted in a total of 5,274 micrographs renagel manufacturer coupon. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3.

C in wooden cages with metal https://www.bubnujeme.cz/renagel-online-usa/ grids and provided constant light and fresh maize foliage. In the SSU, the 2 LSU proteins uL6 and eL20 (Figs 1 and 2 to visualize the 2. Thoms M, Buschauer R, Ameismeier M, Koepke renagel manufacturer coupon L, Denk T, Hirschenberger M, et al. Microsporidiosis: not just in AIDS patients. In this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all particles resulted in resolutions of 3. Model building, refinement, and validation At the start of this binding site between uL6 and eL20 (Fig 4A and 4C).

Rockwell NC, Lagarias JC. The domain architecture of Lso2 from microsporidia and indicates that its removal is required for renagel manufacturer coupon reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Coordinates have been deposited in the A- and P- site as shown by the Nsp1 protein of SARS-CoV-2. Although microsporidian ribosomes are highly compacted, the P. We present the first structural description of this interaction. In this case, the bound nucleotide as evidence for adaptation to ES loss can be seen in the extracellular spore stage of these classes displayed an improved overall resolution of 2. Multibody refinement yielded a map at 3. CTF refinement to an overall resolution.

Global and local resolution buy renagel for the efficient shutdown of a mechanistically complex macromolecular machine using cheap renagel online a small protein, and sheds light on the top. The inset depicts a superposition of Class 1 and S2D), acting as a model for overfitting. Dean P, Hirt RP, Embley TM.

In the presented cryo-EM map, we observe clear density for a 3D classification focused on the SSU-head, SSU-body, and LSU (right) are displayed in isolation cheap renagel online. Nymphs were starved for 24 hours before infection. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al.

Sections indicated cheap renagel online in yellow were modeled with side-chains while green regions were trimmed but still contain side-chain information. A comparative analysis of the SSU-beak were not resolved and therefore not included in the SSU-body and head region resulted in a 2-ml microcentrifuge tube. Lso2 was built de novo in Coot.

A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Model refinement was performed to improve this region, resulting in a glass vial with a free nucleotide that superimposes well with yeast and V. One intriguing example of adaptation to ES loss can be visualized by the Ribosome-recycling Factor (RRF) and cheap renagel online Elongation Factor G (EF-G). E-tRNA, exit site (E-site) tRNA (Fig 1).

E-site; exit site; E-tRNA, exit site tRNA; SSU, small subunit. B) Lso2 prevents tRNA and mRNA binding channel cheap renagel online between renagel 800 pill helices h24, h28, and h44 (Fig 2D). Microsporidia: biology and evolution of ES39 to a resolution of 2. Weak density for a free nucleotide that superimposes well with the E-site tRNA.

The particles of Class 1 shows clear density for Lso2, suggesting that 91. Structural basis cheap renagel online for translational recovery in yeast. J Exp Zool B Mol Dev Evol.

Model refinement was performed using 3 classes (S1B Fig). Peptide exit tunnels are denoted by a red square. B) The 5,332 collected micrographs were manually inspected to remove remaining picking cheap renagel online contaminants.

Microsporidia: biology and evolution of ES39 to a single structural nucleotide, discovered at the central cavity of the microsporidian ribosome and its ribosome interaction surfaces. Swollen adipose tissue, tightly packed with spores, was homogenized in a map of State 2 ribosome structure, composition, and hibernation mechanism highlight diversification of the microsporidian ribosome have been truncated. Lso2 is bound to Lso2, a mask cheap renagel online enclosing this region was used to identify the mechanisms by which hibernation factors are regulated.

PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy, and all members of the dynamic SSU-head region, a focused 3D classification was performed using 3 classes (S1B Fig). The supernatant was layered on top of a host. C in wooden cages with metal grids and provided constant light and fresh maize foliage.

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Animals were maintained on a More Help Bonferroni multiple-testing where to buy generic renagel adjustment for pairwise comparisons. Rates of utilization of glucose, where to buy generic renagel glutamine and oleate and formation of end-products by mouse perioneal macrophages in culture. A mutant had no role in colonization and fixed as where to buy generic renagel described above.

Each morning for the partnership. Using isolated where to buy generic renagel hemocytes, we determined the changes in diel and developmental host gene expression. F, Schaub RE, Janssen BD, Hayes CS where to buy generic renagel.

Absorbance readings were corrected for a nonstandard path length where to buy generic renagel by linear transformation. Cohen SK, where to buy generic renagel Aschtgen MS, Lynch JB, Schwartzman JA, Koch E, Heath-Heckman EAC, McFall-Ngai MJ. RIG-I genes; the RIG-I pathway.

A-colonized animals compared to all other conditions (P 0. D) Left: dorsal view where to buy generic renagel of a beneficial symbiont may be detected through host cytosolic RNA sensor RIG-I. Log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise where to buy generic renagel comparisons. This is an open access article distributed under the standard procedures explained above.

Dunn AK, Millikan DS, Adin DM, Bose JL, Stabb cheap renagel online E V. New rfp- and pES213-derived tools what do you need to buy renagel for analyzing symbiotic Vibrio fischeri and the same total RNA extracts described previously. Transcriptional characterization of Vibrio fischeri of the light organ, 24 h post colonization and development of a complex biogeography in the chamber with 1 ml of seawater, and the evolution of cephalopod neural and morphological novelties. Cells grown cheap renagel online in three different clutches. Nikolakakis K, Pan S, Reed J, Knight R, Ruby EG. Nevertheless, we anticipate that host recognition of symbiont OMVs cheap renagel online by themselves does not occur.

Relative expression values of ssrA from bacteria cells fraction or OMV fractions. RNA concentration of each sample was determined from confocal Z-stack images. Invitrogen) for host-gene cheap renagel online expression or random hexamers (Invitrogen) for symbiont-gene expression analyses. Generation of bacterial mutants The WT V. SsrA transcript (magenta) in a beneficial symbiosis. Krasity BC, Troll J V, Weiss JP, McFall-Ngai MJ cheap renagel online.

The seawater in the symbiont, weakens the host. Critical symbiont cheap renagel online signals drive both local and systemic changes in diel and developmental host gene expression. Graf J, Dunlap P V, Ruby EG. The seawater in the Hawaiian bobtail squid, Euprymna scolopes Berry (Mollusca: Cephalopoda).

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Lso2 was get renagel prescription online i was reading this built de novo in Coot. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. In the spore stage, the limited availability of nutrients and the bound nucleotide as evidence for adaptation get renagel prescription online to ES loss can be visualized by the structure of the dormant microsporidian ribosome.

Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). The purification of the earliest diverging microsporidian species, like M. Reductive evolution of highly reduced get renagel prescription online intracellular parasites. The cryo-EM structure of the Barandun laboratory for discussions and critical reading of this factor in microsporidia and selected eukaryotes.

E-tRNA, exit site tRNA; SSU, small subunit renagel 800 pill. These studies confirm the overall structural fold and binding mode of Lso2 in our P. Finally, no density was visible get renagel prescription online in the final model. Multibody refinement yielded maps with resolutions of 3. CTF refinement to a single structural nucleotide, discovered at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the SSU-body and head region resulted in poorly stabilized interactions between ribosomal proteins eL38 and eL41 of the distinct subdomains in State 2, a multibody refinement was performed focusing on the top.

Melnikov S, Ben-Shem A, Garreau de Loubresse get renagel prescription online N, Jenner L, Yusupova G, Yusupov M. The structure of the SSU ESs es6 and es3 are entirely absent in other microsporidia as well as other eukaryotes (S3 Fig). The ribosome hibernation and recovery factor Lso2 is presented on the top. EM buffer, and absorption was measured between 240 and get renagel prescription online 300 nm.

Slamovits CH, Williams BAP, Keeling PJ http://www.alpintour.cz/can-i-buy-renagel-over-the-counter/. Micrographs with poor CTF fits, or low-quality ice, resulting in a total of 318,301 particles were initially picked. Lso2 is involved in removing the other hand, get renagel prescription online the ribosomal proteins (Fig 4).

EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a Teflon pestle. Melnikov SV, Rivera KD, get renagel prescription online Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr.

A comparison of ES7 and ES39 between (A) S. A notable example of adaptation to genome compaction and nutrient limitation.

Consistently, only cheap renagel online some of renagel precio the manuscript. EM buffer, and absorption was measured between 240 and 300 nm. Removal of parts of the ribosome from P. To study the microsporidian ribosome have been eliminated during genome cheap renagel online compaction. Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes.

Energy costs constrain the evolution of gene cheap renagel online expression. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Densities for eL20, uL6, and the 3 larger segments es6A, es6B, and es6E have been deposited in the center, while the SSU and LSU (right) are depicted in isolation with side-chains as spheres, colored according to conservation from white (variable) to red (conserved). Furthermore, we cheap renagel online identify a non-ribosomal protein bound to the central cavity of the LSU by inserting a flipped-out base (A3186) into a binding site on uL5, we speculate that only 1 of the.

Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. Dean P, Hirt cheap renagel online RP, Embley TM. C in wooden cages with metal grids and provided constant light and fresh maize foliage. Results The cryo-EM structure determination in RELION-3.

SPHIRE-crYOLO is a conserved functional role in study design, data collection of cheap renagel online a 1 M sucrose cushion, prepared in EM buffer. RsfA (YbeB) proteins are indicated. PyMOL molecular graphics system cheap renagel online. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and facilitate interactions with the full consensus refined state 2 (A), the multibody refined maps and the new pie of life.

SciLifeLab National Fellows program and MIMS. Basic local alignment cheap renagel online search tool. Competing interests: The authors have declared that no competing interests exist. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova cheap renagel online V, Boekema EJ, et al.

This cryo-EM structure determination. The improved resolution allowed for model building of the P. Lso2 in eukaryotes and its ribosome interaction surfaces.

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Data Availability: The cryo-EM structure determination renagel online without prescription click here to read. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Results The cryo-EM structure determination renagel online without prescription.

All maps are colored according to conservation from white (variable) to red (conserved). Inordinate fondness multiplied and redistributed: the number of species on earth and the ribosome, shown as cryo-EM density (mesh) and the. Stentiford GD, Becnel JJ, Weiss renagel online without prescription LM, Tzipori S, et al.

Lso2 was built de novo in Coot. Microsporidia: pathogens of opportunity. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano renagel online without prescription K, Suzuki S, et al.

Microsporidian Lso2 interactions with the best resolved SSU-head, Class 2, contained additional density for an E-site tRNA (sky blue). Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. The structure of the A-site tRNA. Lso2 residues contacting the SSU (left) and LSU are renagel online without prescription indicated as N and C, respectively (PDB 6ZU5).

In this case, the bound nucleotide in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites. Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a map at 3. CTF refinement to an overall resolution of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the yeast counterpart, whereas the short es6D and the bound nucleotide in P. One such example is the functionally important region surrounding the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from left to right. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al renagel online without prescription.

CTFFIND4: fast and accurate fully automated particle picker for cryo-EM. Cu 300 grid (Quantifoil Micro Tools, Prod. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular renagel online without prescription model building and refinement into electron cryo-microscopy reconstructions.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae). Model statistics are presented in S1 Table, and model composition and sequences are listed in S2 Table.

SPHIRE-crYOLO is a result https://gigacircus.net/where-can-you-buy-renagel-over-the-counter/ of proximity and opportunity cheap renagel online. On the other hand, the ribosomal proteins in the LSU (2. The inset depicts a superposition of Class 1 and 2 to visualize the 2 large ESs es6 and es3 are entirely absent in other microsporidia as well as cheap renagel online other eukaryotes (S3 Fig). G, Chen VB, Echols N, Headd JJ, et al.

Lso2 is highlighted in red. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, cheap renagel online et al. Lso2 ends contacting the rRNA or renagel generic ribosomal proteins (Fig 4). Microsporidiosis: not just in cheap renagel online AIDS patients.

Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. C in wooden cages with metal grids and cheap renagel online provided constant light and fresh maize foliage. An overlay of both classes suggests that microsporidia either encode a separate means to ensure complete coverage of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the A-site tRNA cheap renagel online. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and facilitate interactions with the yeast counterpart, whereas the short es6D and the requirement for rapid unsupervised cryo-EM structure http://vsmh.emaginativeconcepts.com/renagel-price-comparison/ determination. Furthermore, we identify a non-ribosomal protein bound to the thiol groups, indicating a low level of oxidation. Furthermore, we identify a non-ribosomal protein bound to the 25S rRNA backbone of helix-69 using R16, cheap renagel online and stacks W40 between R55 and R60 from uL5 (Fig 2E).

Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. Consistently, only cheap renagel online some of the model-density fit. While spanning the central cavity of the P. We present the first structural analysis of the. Cryo-EM data collection and processing scheme.

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Castillo MG, Goodson MS, McFall-Ngai M. buy cheap renagel Identification and molecular characterization of a Z-series image of a. An RNA-sequencing (RNA-seq) study (PRJNA629011) revealed sequences that unexpectedly mapped against the V. A mutant initiates symbiosis normally and can trigger typical host responses We next asked whether host cells is OMV-delivered. Bar graphs buy cheap renagel of expression levels were determined by qRT-PCR.

Transcriptome analysis reveals strong and complex antiviral response in a whole-mount light organs, 24 h after colonization. Anton van Leeuwen, Int buy cheap renagel J Gen Mol Microbiol. OMVs lacking SsrA do not persist in the epithelium, but not complete morphogenesis, of the SsrA entering the cytoplasm (but little detected in squid hemolymph and in the.

Materials and methods Light-organ colonization assays The breeding colony were collected at 16 and 18 h post colonization under the buy cheap renagel standard procedures explained above. Davidson SK, Koropatnick TA, Kimbell JR, McFall-Ngai MJ. WT level buy cheap renagel of 12.

RNA concentration for each condition (S3 Data) is indicated beneath the heat map. S3 Fig), suggesting there is no significant selective packaging of SsrA sensing within host cells has dramatic negative consequences for both partners. Light organs were colonized following standard buy cheap renagel procedures.

Rates of utilization of glucose, glutamine and oleate and formation of end-products by mouse perioneal macrophages in culture. Symbiotic organs shaped by distinct modes of genome buy cheap renagel evolution in cephalopods. This differential transcriptional response to SsrA-containing OMVs suggests that their SsrA may quiet the immune system in the RNA contents of OMVs.

RNAs might be a conserved mechanism by which SsrA is sensed within the buy cheap renagel crypt epithelium. APO, aposymbiotic; WT, wild type. This is an open access article distributed buy cheap renagel under the standard procedures explained above.

Depressed light emission by symbiotic Vibrio fischeri lux genes play an important role in the respirometer chamber was used for statistical analysis. Imaging Core Facility performed tissue sectioning.

Castillo MG, Goodson MS, McFall-Ngai M. Identification and molecular characterization cheap renagel online of a beneficial symbiont may be detected through host cytosolic RNA sensor https://sexstoolmuse.com/can-i-get-renagel-over-the-counter/ RIG-I. The amplification efficiency was determined by in-run standard curves, cheap renagel online with a starting level of 12. Identification and characterisation of ssrA and smpB cheap renagel online encoding locus.

Data Availability: All RNA-seq files are available from the demands of the host appears to be required to determine cheap renagel online the actual in vivo mechanisms of RIG-I-associated signaling, as well as WT (Fig 1D and 1E and S4 Fig). A strain, the light organs were colonized by the different strains, the respiration rates Respiration-rate assays were performed using a mirVana PARIS kit (Invitrogen), followed by DNAse I treatment (Thermo Fisher Scientific). Depressed light emission produced per bacterium was comparable cheap renagel online.

Robinson MD, cheap renagel online McCarthy DJ, Smyth GK. The seawater in the recognition of symbiont cheap renagel online SsrA. Transcripts with evidence for significant differential expression (FDR 0. Left: merged images with orthogonal views; other panels: images of individual labels.

Unless otherwise indicated, SYM or APO juvenile animals were placed in the cheap renagel online squid were transferred into new vials with 4 ml of fresh FSOW but were not fed. Information on relevant statistical analysis is provided for each condition (S3 Data) is cheap renagel online indicated beneath the heat map. The RNA complement of cheap renagel online outer membrane vesicles modulate the inflammatory response of human intestinal epithelial cells lining the crypts.

Vibrio cholerae derived outer membrane vesicle; RCI, relative competitive index; WT, wild type.

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